All specimens sent to the laboratory for testing should come with a history of the clinical and gross lesions observed. Multiple specimens from the same farm should be sent because tests on a single specimen may fail to yield the virus. Samples need to be kept frozen during shipping.
Live birds:
The agent is identified by inoculating 9- to 11-day-old embryonated chicken eggs with swabs or tissue specimens, followed by:
Strain virulence can be assessed by evaluation of the intravenous pathogenicity index (IVPI) in 4- to 8-week-old chickens.
Because sera from infected chickens can yield positive antibody tests as early as 3 to 4 days after the first signs of disease appear, serologic tests can be useful to diagnose the disease (see References : Beard 1998).
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1. Definition of Avian Influenza?
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7. HPAI As a Biological Weapon?
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